Fig. 5
From: Targeting oncogenic TERT promoter variants by allele-specific epigenome editing
Viability of Hep-G2 and A-549 after ASEE, analyzed by flow cytometry with Zombie NIR system. Bar plot showing the percentage (%) of dead cells within the Hep-G2 cell population (A) and A-549 cell population (C) carrying all three components (triple-positive for dCas9-10X Suntag vector, DNMT3A-3L vector and sgRNA vector). Bar plot showing the percentage (%) of dead cells within the Hep-G2 cell population B and A-549 cell population D carrying none of the three components (triple negative). A–D Purple and blue color corresponds to cells transfected with TERT sgRNA targeting the mutated (C228T-mut) and wildtype (C228T-wt) allele at C228T position respectively, light green color corresponds to cells transfected with scrambled sgRNA, and orange color corresponds to cells transfected with TERT sgRNA targeting the G alternative allele at rs2853669 SNP position (rs2853669-alt)