Fig. 3

TERT promoter DNA methylation in A-549 after ASEE targeting the alternative allele at rs2853669 SNP. Average allele DNA methylation in A-549 after ASEE with sgRNA targeting the G alternative allele at rs2853669 SNP position. A, C sgRNA rs2853669-alt, on-target alternative G allele orange; B, D off-target reference A allele orange. A, B The x-axis shows the position of CpG sites in the BS1 assay according to the reference sequence used in the analysis and the orange line at positions 237–242 shows the binding site for sgRNA rs2853669-alt. C, D The x-axis shows the position of CpG sites in the BS2 assay according to the reference sequence used in the analysis and the orange line at positions 59–64 shows the binding site for sgRNA rs2853669-alt. A–D Untreated A-549 (UT) are shown in yellow and A-549 transfected with scrambled sgRNA are shown in light green. Error bars are calculated based on at least three independent experiments. Black bar plots show the 1-q value after T Test comparison between A-549 transfected with rs2853669-alt and A-549 transfected with scrambled sgRNA. q values > 1 were set as 1 for visualization purposes. Highly significant CpG sites are indicated by stars (q < 0.05)