Fig. 6

Rspo3 as a tumor suppressor in primary CCA model. A Representative overview of liver tumor load in shNC, shRspo3#1 and shRspo3#2 mice. B Ratios of liver weight to body weight in shNC, shRspo3#1, and shRspo3#2 mice groups. C Representative overview of liver tumor load in oeVector and oeRspo3 mice. D Ratios of liver weight to body weight in oeVector and oeRspo3 mice groups. E, G H&E analysis of the number of primary CCAs in shNC, shRspo3#1, and shRspo3#2 mice groups. F, H H&E analysis of the number of primary CCAs in oeVector and oeRspo3 mice groups. I Kaplan–Meier curves showing overall survival in the shNC, shRspo3#1, and shRspo3#2 primary CCA model mice groups. J Kaplan–Meier curves showing overall survival in the oeVector and oeRspo3 primary CCA model mice groups. K, L Immunohistochemical staining of cytokeratin 19 in primary CCA model. M–P. Immunohistochemical staining was performed to detect proliferation of primary CCA tissue. Percentages of Ki67 positive cells were analyzed