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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Upregulation of RSPO3 via targeted promoter DNA demethylation inhibits the progression of cholangiocarcinoma

Fig. 5

RSPO3 as a CCA suppressor by targeting methylation/demethylation of RSPO3 in vivo. A Overview of tumors in xenografts of RSPO3 targeted methylated and control cells in vivo (n = 8). B, C Tumor volume and end-stage tumor weight after injection of TFK1 cells transfected with sgRNA-dCas9DNMT3a and sg-RSPO3-dCas9DNMT3a. D Overview of tumors in xenografts of RSPO3 targeted demethylated and control cells in vivo (n = 8). E, F Tumor volume and end-stage tumor weight after injection of QBC939 cells transfected with sgRNA-dCas9TET1CD and sg-RSPO3-dCas9TET1CD. G–L. Immunohistochemical staining was performed to detect proliferation and infiltration of tumor tissue. Percentages of Ki67 and N-cadherin positive cells were analyzed

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Clinical Epigenetics

ISSN: 1868-7083

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