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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Upregulation of RSPO3 via targeted promoter DNA demethylation inhibits the progression of cholangiocarcinoma

Fig. 3

DNMT3a-mediated RSPO3 DNA hypermethylation promotes CCA growth. A–D Overexpression of DNMT1, DNMT3a, DNMT3b and DNMT3l in TFK1 cells confirmed by RT-qPCR. Data are shown after normalization to β-actin. E Expression of RSPO3 in DNMT1, DNMT3a, DNMT3b and DNMT3l overexpression TFK1 cells. Data are shown after normalization to β-actin. F An illustration of targeted methylation by sgRNA-dCas9DNMT3a. G Differential DNA methylation targeting RSPO3 promoter methylation in CCA cell lines TFK1 and EGI1. H, I RSPO3 expression after targeted RSPO3 promoter methylation in CCA cell lines TFK1 and EGI1. J, K Cell proliferation after methylation of the targeted RSPO3 promoter in the CCA cell lines TFK1 and EGI1 was performed using Cell Counting Kit-8 (CCK-8) assay. L–N Cell proliferation after methylation of the targeted RSPO3 promoter in the CCA cell lines TFK1 and EGI1 was performed using clone formation assay

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Clinical Epigenetics

ISSN: 1868-7083

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