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Fig. 5 | Clinical Epigenetics

Fig. 5

From: Abnormal expression of PRKAG2-AS results in dysfunction of cardiomyocytes through regulating PRKAG2 transcription by interacting with PPARG

Fig. 5

PRKAG2-AS interacting proteins. A Two strategies were employed to screen for PRKAG2-AS interacting proteins. B The expression of PRKAG2-AS by adenovirus was confirmed by qRT-PCR. C Successful RIP was confirmed by the result that Flag antibody could enrich PRKAG2-AS relative to the IgG control. D Biotin-labeled antisense oligos were able to purify PRKAG2-AS. E RIP and biotin-antisense purification identified 304 and 162 proteins, respectively, with 58 proteins appearing in both sets. F The functional relationships between the 58 identified PRKAG2-AS binding proteins were analyzed through STRING and Cytoscape

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Clinical Epigenetics

ISSN: 1868-7083

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