Fig. 1
PRKAG2b and PRKAG2d were transcriptionally regulated by nuclear PRKAG2-AS. A A long noncoding RNA called PRKAG2-AS is found within the promoter region of the PRKAG2 gene, which has five isoforms resulting from various transcription starting sites. B RT-PCR analysis of the expression profiles of the 5 PRKAG2 isoforms in AC16, HCAEC, 293Ā T, and HUVEC cells. C-D RNA nucleoplasmic separation of AC16 cells and qRT-PCR revealed the nuclear localization of PRKAG2-AS. U6 and GAPDH were used as controls for nuclear and cytoplasmic localization, respectively. E Fluorescence in situ hybridization using 3 biotin-oligos targeting PRKAG2-AS showed its predominant localization within the nucleus. F Knockdown of PRKAG2-AS in AC16 cells by siRNA. G-H The expression of PRKAG2b and PRKAG2d was unaffected by the siRNA-mediated knockdown of PRKAG2-AS. (I) PRKAG2-AS was knocked down in the nucleus by antisense oligonucleotides. J-K The mRNA levels of PRKAG2b and PRKAG2d were significantly reduced in cells subjected to knockdown of nuclear PRKAG2-AS by antisense oligonucleotides