Fig. 4

HDAC3 deficiency in AT2 cells alleviates pulmonary fibrosis in vivo and in vitro through suppression of EMT. A Histological analyses showing H&E staining, masson staining, PSR staining and Ashcroft score for fibrosis of BLM-treated mice at day 21 in HDAC3-C and HDAC3-CKO mice. (× 200, n = 5). B The relative mRNA expression of Col 1, α-SMA, E-cadherin, N-cadherin and vimentin in HDAC3-C and HDAC3-CKO mice in the presence of saline or BLM for 21d. (n = 5). C Representative Western blot images and the corresponding quantitative results for E-cadherin, N-cadherin and vimentin in HDAC3-C and HDAC3-CKO mice in the presence of saline or BLM for 21d. (n = 5). D The relative mRNA expression of E-cadherin, N-cadherin and vimentin in PBS-treated or TGF-β1 treated primary AT2 cells from HDAC3-C and HDAC3-CKO mice for 72 h. (n = 5). E Representative Western blot images and the corresponding quantitative results for E-cadherin, N-cadherin and vimentin in HDAC3-C and HDAC3-CKO mice in PBS-treated or TGF-β1 treated primary AT2 cells from HDAC3-C and HDAC3-CKO mice for 72 h. (n = 5). (*P < 0.05, **P < 0.01, ***P < 0.001, ^nsP > 0.05)