Fig. 2

HDAC3 is upregulated in lung tissues and AT2 cells from mice with BLM-induced pulmonary fibrosis. A Histological analyses of H&E staining, masson staining, PSR staining and Ashcroft score for fibrosis in BLM-treated mice at day 0, 7, 14 and 21 post-treatment (× 200, n = 5). B Relative mRNA expression of HDAC3, Col 1 and α-SMA in lung tissues from BLM-treated mice at day 0, 7, 14 and 21 (n = 5). C Representative Western blot images and corresponding quantitative results for HDAC3, Col 1 and α-SMA in lung tissues from BLM-treated mice at day 0, 7, 14 and 21 (n = 5). D HDAC3 activity in lung tissues from saline and BLM-treated mice (2.5 mg/kg for 21d; n = 5). E Representative images showing HDAC3 and ABCA3 immunofluorescence colocalization staining and quantitation in lung tissues from BLM-treated mice at day 21 (× 400, n = 5). (*P < 0.05, **P < 0.01, ***P < 0.001, ^nsP > 0.05)