Fig. 1

EZH2 is overexpressed in RMS and inhibition leads to a decrease in cell proliferation. A Photomicrograph of tissue microarray cores, showing examples of EZH2 protein expression in fusion negative RMS (FNRMS) and fusion positive RMS (FPRMS) samples scored as 0 (less than 5% positively stained cells), 1(weak), 2 (moderate), 3 (high). Scale bars = 50 μm. B EZH2 transcript is overexpressed in RMS tissue samples and cell lines. Datasets of RMS patient (ITCC n = 101, Barr n = 58 [2, 29]) and cell lines (Versteeg n = 22), were compared to skeletal muscle (Asmann n = 40) [30] using R2 Genomics Analysis and Visualization Platform [30]. Results are visualised as boxplots with a log2 transformation C GI50 after GSK343 treatment for RMS cell lines, HFF-1, and HS-SY-II. D Cell proliferation of RD and RH30 treated with indicated concentrations of GSK343 or ATRA for 6 days