Fig. 2

KS1 mouse signature has high sensitivity and specificity, as determined by cross-validation. A PCA at 1599 signature sites in only vehicle-treated samples (n = 13 WT; n = 10 KS1). B Heatmap of signature sites, showing even split between hyper- and hypomethylation, as well as clustering of samples by genotype. Dendrograms generated with Euclidean clustering. DNA methylation (Beta values) is normalized and ranges from − 2 to 2  C Leave-one-out cross-validation shows all samples correctly clustering by genotype. Samples, when left out and used for cross-validation, are more strongly correlated with samples of the genotype at signature sites