Fig. 4

QC6352 combined with SSK1 inhibited tumor growth in vivo. A The workflow of the “one-two punch” therapeutic mouse model. B Spider plots of tumor growth in each group. Each line represented one mouse (n = 8). C Representative images of xenograft tumors with the indicated treatment at the end point (n = 8). D Scatter plot of xenograft tumors with the indicated treatment at the end point (n = 8). E The body weight of mice in each group was recorded daily and summarized as indicated (n = 8). F Representative terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining in tumor tissues derived from the indicated group. Scale bar, 50 μM. G Representative immunofluorescence staining for H3K9me3 in tumor tissues derived from the indicated group. Scale bar, 50 μM. H The tumor tissues derived from each group were lysed, followed by immunoblotting analysis (n = 3). I The tumor tissues derived from each group were lysed, followed by ELISA analysis (n = 3). J MDA levels in tissues derived from the indicated group were determined by an MDA kit. **P < 0.01 and ***P < 0.001 from two-tailed unpaired Student’s t tests. Data represent the mean ± SD (n = 8 mice per group)