Fig. 2

QC6352 triggered senescence in gastric cancer harboring the TP53 mutation. A Representative images of SA-β-Gal staining in gastric cancer cells treated as indicated. Scale bar, 20 μm. B Immunofluorescence staining for H3K9me3 in cells before and after QC6352 (20 nM) treatment. Scale bar, 20 μm. C Gastric cancer cells were treated with DMSO or QC6352 (20 nM) and the cell cycle profile was obtained by propidium iodide (PI) staining and fluorescent-activated cell sorting (FACS) analysis. D Gastric cancer cells were treated with gradient doses of QC6352 (10 nM and 20 nM), followed by immunoblotting. E Gastric cancer cells were treated with 20 nM QC6352, followed by enzyme linked immunosorbent (ELISA). F Gastric cancer cells were treated with gradient doses of QC6352 (10 nM and 20 nM), followed by malondialdehyde (MDA) detection. *P < 0.05, **P < 0.01, and ***P < 0.001. P values were determined by two-tailed unpaired t test. Data were presented as mean ± SEM of three independent experiments