Table 2 Current methods available for chromosome accessibility sequencing
From: Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity
Technique | Technical features | Designed by |
|---|---|---|
scATAC-seq | scATAC-seq enables sequencing of chromatin accessibility of each individual cell; technical complexity; data complicated and high costs | [54] |
scMNase-seq | scMNase-seq sequences and analyzes chromatin structure and accessibility in individual cells by enzymatic cleavage of chromatin by micronucleases (MNases); limitations of technical complexity and fragmentation; high cost | [56] |
sciATAC | sciATAC is a DNA transposase-based single-cell sequencing technology for analyzing chromatin accessibility in single cells; introduced the strategy of combinatorial indexing; limitations of technical complexity and fragmentation; high cost | [57] |
SNuBar-ATAC | SNuBar-ATAC is a technology for measuring single-cell chromatin accessibility and gene expression with high resolution, integrated multiple measurements and microfluidics; data complicated and high costs | [58] |
sciMAP-ATAC | SNuBar-ATAC is a technology for measuring single-cell chromatin accessibility and gene expression with high resolution, integrated multiple measurements and microfluidics; limitations of technical complexity and fragmentation; high cost | [59] |
scGET-seq | scGET-seq is a hybridized transposase-based sequencing of single-cell genomes and epigenomic transposases, enabling comprehensive probing of open and closed chromatin and simultaneous documentation of underlying genomic sequences | [60] |
HyDrop-ATAC | HyDrop-ATAC is a technology for measuring single-cell chromatin accessibility and gene expression with high resolution, direct sequencing and capture of dynamic information (introducing special fluorescent markers); limitations of technical complexity and fragmentation; high cost | [61] |