Fig. 3

Unsupervised clustering analyses of the 28 cfDNA pooled samples. A Hierarchical clustering and heatmap showing the methylation levels across all samples for the 376 DMPs. B Hierarchical clustering and heatmap showing the methylation levels across all samples for the 26 candidate biomarkers. Each column represents one pool, while rows correspond to CpG sites. Dendrograms were computed and reordered using Euclidean distance and a complete clustering agglomeration. Methylation levels are expressed as beta-values ranging from 0 (blue, unmethylated) to 1 (red, fully methylated)