Fig. 3
Intra-plantar inflammation in early life is not associated with long-term changes in Fkbp5 mRNA expression. A Primed animals received a s.c. plantar injection of an inflammatory agent at postnatal day 5, and all animals received an injection of prostaglandin E2 at postnatal day 38. N = 8/8. Two-way ANOVA factor treatment, Day 5 to Day 9, F1,14 = 71.9, P < 0.0001; Day 41 to Day 52, F1,14 = 7.3, P < 0.05. Control vs primed. B There was no change in Fkbp5 mRNA expression in the spinal cord or the brain 39 days after carrageenan injection (i.e. before prostaglandin E2 injection). mRNA levels were quantified by RTqPCR. N = 8/8. Data were normalised to control