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Fig. 8 | Clinical Epigenetics

Fig. 8

From: METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma

Fig. 8

METTL3 modulates the H3K36me3 level by mediating m6A modification of SETD2 mRNA. A Overlaps of m6A sites with histone modification sites in A549 cells. B VGF enrichment around H3K36me3 ChIP-seq peak centers in A549 cells. C ChIP‒qPCR analysis of H3K36me3 at VGF. D The expression of SETD2 mRNA was verified by qRT‒PCR. E VGF mRNA was analyzed in A549 cells after actinomycin D treatment. F Predicted m6A sites in the CDS and 3'UTR sequence of SETD2 mRNA. G The m6A levels of m6A sites of SETD2 mRNA in A549 cells were analyzed by MeRIP-qPCR. H Luciferase activity was measured using a dual luciferase assay. I Schematic showing the functional and molecular mechanisms of METTL3 in LUAD. Bar = mean ± SD. *p < 0.05, **p < 0.01

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Clinical Epigenetics

ISSN: 1868-7083

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