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Fig. 7 | Clinical Epigenetics

Fig. 7

From: METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma

Fig. 7

METTL3-mediated m6A modification of VGF mRNA promotes LUAD progression. A, B CCK8 (A) and EdU (B) assays were performed to assess cell viability and proliferation. C Flow cytometric analysis of the cell cycle. D Colony formation assays. E Scratch assay experiments on A549 cells. F, G Cell migratory and invasive abilities in A549 cells. H Gross appearance (left), tumor volume (middle), and tumor weight (right) of each group. I IHC staining results (METTL3, VGF, Ki-67). J Western blot results for METTL3, VGF, p-PI3K, PI3K, p-AKT, AKT, mTOR and p-mTOR. K Western blot was used to detect the effects of LY294002 and Rapamycin on the protein expression of p-PI3K and p-mTOR in A549 cells, respectively. L Western blot results of METTL3, SETD2, and H3 proteins

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Clinical Epigenetics

ISSN: 1868-7083

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