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Fig. 5 | Clinical Epigenetics

Fig. 5

From: METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma

Fig. 5

METTL3 regulates the expression of VGF via m6A modification. A Validation of METTL3 expression levels in the cDNA microarray by qRT‒PCR. B, C qRT‒PCR (B) and western blot (C) measurement of METTL3 expression in A549 and HBE cells. D Correlations between the expression of METTL3 and VGF in LUAD tissues. E Relative VGF expression in LUAD and adjacent noncancerous tissues in the TCGA. F Correlations between the expression of METTL3 and VGF in TCGA-LUAD. G, H The expression of METTL3 and VGF was verified by qRT‒PCR (G) and western blot (H). I The total m6A level of A549 and HBE cells. J The total m6A level of A549 cells after METTL3 overexpression and knockdown. K Prediction results of VGF mRNA in the SRAMP website and schematic photo of CDS-WT and CDS-mutant in VGF mRNA (left), VGF m6A modification levels in A549 cells determined using MeRIP-qPCR (right). L Luciferase activity was measured using a dual luciferase assay. Bar = mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001

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Clinical Epigenetics

ISSN: 1868-7083

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