Fig. 3From: METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinomaVGF knockdown repressed the malignant phenotype of LUAD cells in vitro. A The efficiency of VGF knockdown was verified by qRT‒PCR. B Western blot analysis of VGF, PI3K, phosphorylated PI3K (p-PI3K), AKT, p-AKT, p-mTOR and mTOR protein expression. C-D CCK8 (C) and EdU (D) assays were used to test the effect of VGF on LUAD cell viability. E Cell colony formation assays. F Flow cytometric analysis of the cell cycle. G Scratch assay experiments on A549 cells. H-I Cell migratory (H) and invasive (I) abilities were detected using transwell assays in A549 cells. Bar = mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001Back to article page