Fig. 1

Screening of key biomarkers of LUAD. A Principal component analysis (PCA) analysis of the chromatin property data between LUAD tissues and normal lung tissues. B Heatmap showing affinities for differentially bound sites. C Volcano plot of differential peak expression analysis. Significantly differentially expressed peaks are denoted by red circles. D Distribution of promoter, exons, introns, and intergenic regions. E Line plot shows the chromatin accessibility in LUAD tissues and normal lung tissues around the TSS. F Volcano plot of differentially expressed genes. G Gene Ontology biological process and KEGG enrichment analysis. H Volcano plot for differential gene expression between LUAD cells and HBE cells. I Quantitative real-time polymerase chain reaction (qRT-PCR) verification of TBX4, C1QTNF7, HMGA2, BUB1B, and VGF gene expression. J mRNA density coverage. CDS: coding sequences. K Integrative Genomics Viewer (IGV) tracks displaying MeRIP-seq read distributions in VGF. Bar = mean ± standard deviation (SD). **p < 0.01