Table 2 Key characteristics of cfDNA isolation kits
Kit name | Manufacturer | Input volume (ml) | Elution volume (µl) | Clean-up type | Protocol time (min)b | Automation |
|---|---|---|---|---|---|---|
Maxwell RSC ccfDNA Plasma | Promega | 0.2–1 | 60 | Magnetic beads | 70 | Yes (Maxwell RSC) |
QIAamp Circulating Nucleic Acid | QIAGEN | 1–5 | 20–150 | Spin column | 90 | Partly (clean-up QIAcube) |
QIAamp MinElute ccfDNA Mini | QIAGEN | 1–4a | 20–80 | Magnetic beads + spin column | 70 | Partly (clean-up QIAcube) |