Table 1 Key characteristics of bisulfite conversion kits
Kit name | Manufacturer | Input amount (ng) | Optimal input amount (ng) | Input volume (µl) | Elution volume (µl) | DNA protection | Conversion temperature (°C) | Conversion time (min) | Protocol time (min)c | Automation |
|---|---|---|---|---|---|---|---|---|---|---|
EpiJET Bisulfite Conversion | Thermo Scientific | 0.05–2000 | 200–500 | 1–20 | 10–20 | No | 98 + 60 | 160 | 220 | No |
EpiTect Plus DNA Bisulfite | QIAGEN | 1–2000 | – | 1–20 (40a) | 10–15 | Yes (carrier RNA) | 95 + 60 | 300 | 370 | Partly (clean-up QIAcube) |
EZ DNA Methylation-Direct | Zymo Research | 0.05–2000 | 200–500 | 1–20 | 10b | No | 98 + 64 | 218 | 260 | No |
Imprint DNA Modification | Sigma-Aldrich | 0.1–1000 | 50–200 | 1–24 | 8–20 | Yes (BSA solution) | 65 | 90 | 155 | No |
Premium Bisulfite | Diagenode | 0.1–2000 | 200–500 | 1–20 | 10b | No | 98 + 60 | 68 | 115 | No |