Fig. 8
The effect of TFAM expression in induction of a premature senescent phenotype in FDR APCs. a–c TFAM, CDKN1A, and ZMAT3 mRNA expression levels were measured by qPCR and normalized to RPL13A expression in CTRL TFAM-silenced APCs (CTRL siRNA-TFAM) (n = 6). Values are presented as relative-fold change (RFC). Statistical significance between the two groups was determined by paired Student’s t-test (**p value < 0.01, and ***p value < 0.001 vs CTRL). d–f CDKN1A, ZMAT3, and TFAM mRNA expression levels were measured by qPCR and normalized to RPL13A expression in CTRL APCs exposed to (CTRL H2O2) or not to H2O2 (n = 6). Values are presented as relative-fold change (RFC). Statistical significance between the two groups was determined by paired Student’s t-test (***p value < 0.001 vs CTRL). (g, h) CDKN1A, and ZMAT3 mRNA expression levels were measured by qPCR and normalized to RPL13A expression in CTRL APCs exposed to H2O2 (CTRL H2O2) and silenced for TFAM gene (CTRL H2O2 siRNA-TFAM) (n = 6). Values are presented as relative-fold change (RFC). Statistical significance between the two groups was determined by paired Student’s t-test (***p value < 0.001, and **p value < 0.01 vs CTRL H2O2)