Fig. 7

Mitochondrial alterations and the development of premature senescence in FDR APCs. a, b Correlation analysis between the percentage of SA-β-gal-positive cells and mtDNA content (p value = 0.0007), and TFAM mRNA levels (p value = 0.0327) in FDR and CTRL APCs. The Spearman's rank correlation test was used for correlation analysis and statistical significance assessment. c–e TFAM, and CYTB mRNA expression levels were measured by qPCR and normalized to RPL13A and TBP expression, respectively, in FDR APCs treated with a combination of Dasatinib (D) and Quercetin (Q) (FDR D + Q) (n = 6). Values are presented as relative-fold change (RFC). d mtDNA content in the APCs from FDR and FDR D + Q (n = 6) was measured by qPCR. Values are presented as relative-fold change (RFC). c–e. Statistical significance was determined by paired Student's t-test (*p value < 0.05, and **p value < 0.01 vs FDR)