Fig. 6

TFAM knockdown causes reduction in adipogenic potential of CTRL APCs. a On day 15 of the differentiation process, TFAM mRNA expression levels were measured by qPCR and normalized to RPL13A expression in CTRL adipocytes (Adipo CTRL) and CTRL TFAM-silenced adipocytes (Adipo CTRL-siRNA) (n = 5). Values are presented as relative-fold change (RFC). b The expression of adipogenic marker genes PPARγ, GLUT4, and ADIPOQ mRNA levels was measured by qPCR and normalized to RPL13A expression in Adipo CTRL and Adipo CTRL-siRNA (n = 5). Values are presented as relative-fold change (RFC). c Quantification of Oil Red O staining (n = 6). d The mtDNA content was measured by qPCR in Adipo CTRL and Adipo CTRL-siRNA (n = 6). Values are presented as absolute units (AU), and their distribution within each group is represented by box plots. e ATP6, CYTB, CO2, and ND1 mRNA expression levels were measured by qPCR and normalized to TBP expression in Adipo CTRL and Adipo CTRL-siRNA (n = 5). Values are presented as relative-fold change (RFC). a, b, d, and e Statistical significance was determined by paired Student’s t-test (*p value < 0.05, **p value < 0.01, and ***p value < 0.001 vs Adipo CTRL). c Statistical significance between the two groups was determined by unpaired Mann–Whitney (*p value < 0.001 vs Adipo CTRL)