Fig. 4

TFAM knockdown in CTRL APCs decreases mtDNA content and mitochondrial-encoded gene transcription. a TFAM mRNA expression levels were measured by qPCR and normalized to RPL13A expression in CTRL APCs, and CTRL APCs transfected with TFAM-specific siRNA (CTRL siRNA-TFAM) (n = 7). Values are presented as relative-fold change (RFC). b, c TFAM protein levels were assessed by western blot in CTRL and CTRL siRNA-TFAM APCs. Vinculin served as a loading control. Figure b shows a representative blot (CTRL, n = 3; CTRL siRNA-TFAM, n = 3); Figure c shows the densitometric analysis of proteins bands (CTRL, n = 6; CTRL siRNA-TFAM, n = 6). d The mtDNA content in CTRL and CTRL siRNA-TFAM (n = 4) was measured by qPCR. Values are presented as relative-fold change (RFC). e ATP6, CYTB, CO2, and ND1 mRNA expression levels were measured by qPCR and normalized to TBP expression in CTRL and CTRL siRNA-TFAM (n = 5). Values are presented as relative-fold change (RFC). f ATP concentrations quantified in APCs from CTRL and CTRL siRNA-TFAM (n = 5). a, c, d, and e Statistical significance was determined by paired Student’s t tests (*p value < 0.05, **p value < 0.01, and ***p value < 0.001 vs CTRL). f Statistical significance was determined by unpaired Student's t tests (**p value < 0.01 vs CTRL)