Fig. 3

Epigenetic characterization of TFAM promoter in CTRL and FDR APCs. a, b NRF1 and NRF2 mRNA expression levels were measured by qPCR and normalized to RPL13A expression in APCs from FDR (n = 7) and CTRL (n = 9). Values are presented as absolute units (AU). c A schematic representation of the CpG island (from − 564 to − 20 bp from the TSS) on the TFAM promoter identified by the EMBOSS CpGplot software. d Quantification of the DNA methylation levels at three specific CpG sites (− 495, − 438, and − 429) and the total region (Tot Reg) in APCs from FDR (blue bars) and CTRL (red bars). e, g H3K27ac and H3K27me3 enrichment on the TFAM promoter measured by ChIP-qPCR in subcutaneous APCs from FDR (n = 7) and CTRL (n = 9). f Correlation analysis between H3K27ac and H3K4me3 enrichment in FDR and CTRL APCs. The Pearson correlation test was used for correlation analysis and statistical significance assessment (p value = 0.0218). (a, b, e, and g) Data are shown as boxplots (min–max). a, b, d, e, and g Statistical significance between the two groups was determined by unpaired Student’s t test (*p value < 0.05 vs CTRL)