Fig. 2

H3K4me3 promoter enrichment associates with reduced TFAM mRNA/protein levels in FDR APCs. a H3K4me3 enrichment at the TFAM promoter was measured by ChIP-qPCR in subcutaneous APCs from FDR (n = 7) and CTRL (n = 9). ChIP data were calculated as a percentage relative to the INPUT DNA. b TFAM mRNA expression levels were measured by qPCR and normalized to RPL13A expression in APCs from FDR (n = 7) and CTRL (n = 9). Values are presented as absolute units (AU). c, d TFAM protein levels were assessed by western blot in APCs from FDR and CTRL subjects from our study cohort. Vinculin (VCL) served as a protein loading control. The western blot analysis and densitometric measurement of the bands in 4 CTRL and 4 FDR individuals are shown in Figures c and d, respectively. e The mtDNA content in subcutaneous APCs from FDR (n = 7) and CTRL (n = 9) was determined by qPCR. Values are presented as absolute units (AU). f Correlation analysis between TFAM mRNA levels and mtDNA content in FDR and CTRL APCs. The Spearman’s rank correlation test was used for correlation analysis and assessment of statistical significance (p value = 0.011). g CYTB, CO2, ATP6, and ND1 mRNA expression levels were measured by qPCR and normalized to TATA-Box binding protein (TBP) expression in APCs from FDR (n = 7) and CTRL (n = 9). Values are presented as absolute units (AU). h ATP concentrations quantified in APCs from FDR (n = 7) and CTRL (n = 9). a, b, e, g, and h Data are shown as boxplots (min–max). a, b, d, e, g, and h Statistical significance between the two groups was determined by unpaired Student’s t-test (*p value < 0.05, **p value < 0.01, and ***p value < 0.001 vs CTRL)