Fig. 1

Differential H3K4me3 enrichment in FDR and CTRL subcutaneous preadipocytes. a The average genome-wide histone enrichment, calculated by MACS near TSS (± 1000 bp), was measured for all known genes for each individual H3K4me3 and illustrated for individual samples [SVF (blue)]. The relative-fold enrichment was calculated by the MACS algorithm, which was considered for the background signal by comparing the ChIP peaks within an individual study sample to its own 10% INPUT DNA control by looking for reading orientation and mapping density that represent histone (H3K4me3) binding. b The pie charts generated from the ChIp-Seq peaks annotation show the distribution of the peaks among the genomic features in CTRL and FDR groups. Peak enrichment test using a binomial p value to compare relative peak enrichment across genomic regions of different categories (e.g., upstream promoter region, 5′-UTR, 3′-UTR) is shown in the figure. c The figure shows a Venn diagram of the overlapping genes among the three most enriched GO terms