Evaluation of pediatric epigenetic clocks across multiple tissues

Clinical Epigenetics

Table 3 NeoAge clock estimations for post-menstrual age (PMA) and post-natal age (PNA)

Tissue	Array	Birth term	GA (weeks)	N	NeoAge PMA^ỻ				Knight
Tissue	Array	Birth term	GA (weeks)	N	\(r\)*	Median error^†	Median res^§	Mean diff^‡	\(r\)*	Median error^†	Median res^§	Mean diff^‡
Post-menstrual age (weeks) estimation
Blood spot	450K	Term	39.36 ± 1.91	253	0.18	4.72	0.29	− 4.71	0.35	1.11	0.96	− 0.08
	EPIC	Term	38.73 ± 1.60	176	0.27	3.08	0.21	− 2.85	0.48	0.89	0.91	− 0.32
	EPIC	Preterm	25.65 ± 1.27	272	0.27	8.31	0.29	8.53	0.57	2.52	1.01	2.52

Tissue	Array	Birth term	GA (weeks)	N	NeoAge PMA^ỻ				Lee
Tissue	Array	Birth term	GA (weeks)	N	\(r\)*	Median error^†	Median res^§	Mean diff^‡	\(r\)*	Median error^†	Median res^§	Mean diff^‡
Placenta	EPIC	Term	38.79 ± 1.18	121	0.16	1.91	0.39	− 2.01	0.36	0.88	0.76	− 0.32
		Preterm	25.68 ± 1.25	399	0.15	9.88	0.36	10.13	0.6	2.50	0.93	2.59

Tissue	Array	Birth term	GA (weeks)	N	NeoAge PMA^ỻ				Knight
Tissue	Array	Birth term	GA (weeks)	N	\(r\)*	Median error^†	Median res^§	Mean diff^‡	\(r\)*	Median error^†	Median res^§	Mean diff^‡
Buccal cells	EPIC	Term	38.13 ± 1.09	72	0.44	0.98	0.44	− 0.87	0.25	11.01	0.59	− 10.96
Buccal cells^¶	EPIC	Preterm	26.63 ± 1.89	480	0.99	0.71	0.23	− 0.78	− 0.06	2.00	0.74	− 0.75

Tissue	Array	Birth term	Age (weeks)	N	NeoAge PNA (weeks)				PedBE (weeks)
Tissue	Array	Birth term	Age (weeks)	N	\(r\)*	Median error^†	Median res^§	Mean diff^‡	\(r\)*	Median error^†	Median res^§	Mean diff^‡
Post-natal age (weeks) estimation
Buccal cells	EPIC	Term	0.52 ± 0.75	72	− 0.02	7.35	0.65	7.44	0.12	2.66	2.53	2.30
Buccal cells^¶	EPIC	Preterm	12.14 ± 4.52	480	1	1.00	0.20	− 1.19	0.62	4.46	4.70	3.38

*Spearman correlation between predicted epigenetic age and observed gestational age/chronological age
^†Median of the absolute difference between the predicted age and the observed age (weeks for gestational age and years for chronological age)
^§Median of the absolute residuals from regressing the predicted epigenetic age onto the observed age (weeks for gestational age and years for chronological age)
^‡Mean of the signed difference between the predicted age and the observed age (weeks for gestational age and years for chronological age)
CBMC Cord blood mononuclear cells, GA gestational age, PBMC Peripheral blood mononuclear cells
^¶Training data used in developing NeoAge clock
^ỻThe observed PMA was calculated as the sum of gestational age and the time elapsed after birth in weeks

ISSN: 1868-7083