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Fig. 1 | Clinical Epigenetics

Fig. 1

From: Introduction of a multiplex amplicon sequencing assay to quantify DNA methylation in target cytosine markers underlying four selected epigenetic clocks

Fig. 1

Distribution of reads across all cytosine targets and all sequencing runs performed in a repeatability study for Ion AmpliSeq (A) and SureSelect (B) technologies, using raw (left) and normalized (right) read depth. The green line on the raw read depth graphs indicates the minimum number of reads set to 50, while the green line on the normalized read depth graphs shows an expected depth of 0.00065 for Ion AmpliSeq and 0.00060 for SureSelect, assuming a perfect distribution of reads between targets. The X-axis shows the target cytosines in the order given in Additional file 1: Table S1

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Clinical Epigenetics

ISSN: 1868-7083

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