Cross-platform comparisons for targeted bisulfite sequencing of MGISEQ-2000 and NovaSeq6000

Clinical Epigenetics

Table 2 The summary QC of the data of MGISEQ-2000 and NovaSeq6000

Data type	Library type	Sequencer	High-quality reads ratio (%)	Mapping ratio (%)	On-target ratio (%)	Uniformity ratio (%)
mLib-MGISEQ	mLib	MGISEQ-2000	86–88	46–49	75–79	56–60
iLib-NovaSeq	iLib	NovaSeq6000	86–89	49–52	70–75	59–64
mLib-NovaSeq	mLib	NovaSeq6000	86–88	48–52	72–75	59–64

“Library Type” represented the kit used to prepare libraries. “Sequencer” represented the sequencer to generate data. High-quality reads ratio” demonstrated the ratio of high-quality reads (phred > 30). “Mapping Ratio” represented the ratio of reads that aligned to human genome. ‘On-target Ratio’ represented the ratio of mapping reads which were amplified by panel primers and located in targeted genome regions. ‘Uniformity Ratio’ demonstrated the uniformity of panel targeted priming, which was calculated using the ratio of CpGs whose coverages were larger than 25% median coverage. The values before “–” represented the minimum values, while those after “–” represented the maximum values

ISSN: 1868-7083