Fig. 5

MGISEQ-2000 exhibited matching clinical performance with NovaSeq6000. A The distribution of insert size of sequencing data of clinical cfDNA samples. The x-axis represented the insert size of sequencing data; the y-axis represented the distribution of insert size; colors represented different sequencers. B The percentage of alignments in different insert size intervals. Filled colors represented the intervals of insert size. C Scatter plot showing minor variances of AMFs (upper) and CpG methylation levels (below) of mLib-MGISEQ and iLib-NovaSeq. The y-axis represented the deviation values of mLib-MGISEQ and iLib-NovaSeq, and the x-axis represented the mean values. Colors represented the density of points. Black points represented the outliers. MSE represented the mean squared error and bias represented the mean error. D The prediction scores of 24 cfDNAs. The x-axis represented the 24 cfDNAs; the y-axis represented the prediction score with the in-house model; the filled colors represented sequencers. The prediction scores of mLib-MGISEQ were positively correlated with those of iLib-NovaSeq. The blue dashed line represented the threshold of 0.8952 for NovaSeq6000 data and the orange dashed line represented the threshold of 0.8956 for MGISEQ-2000 data. E The receiver operating characteristic curves (ROCs) of down-sampling iLib-NovaSeq6000 data (left), and mLib-MGISEQ-2000 data (middle). The colors on the left and middle panels represented the size of the down-sampling data. The table on the right represented the down-sampling data sizes and their corresponding coverages and area under curves (AUCs) and the numbers in brackets represented AUCs ± 95% CI