Fathers’ preconception smoking and offspring DNA methylation

Clinical Epigenetics

Table 2 A and B. CpG sites associated with father’s smoking at genome-wide significance (FDR < 0.05)

Fathers’ smoking	CpG	Coefficient*	Average**	SD	Adj.P***	Gene	Location****
A: Any preconception smoking onset	cg00870527	− 0.024	0.5	0.07	0.028	PRR5	N_Shelf
	cg08541349	− 0.012	0.88	0.023	0.028	CENPP	OpenSea
B: Fathers’ smoking onset before age 15	cg23021329	0.015	0.27	0.021	0.026	TLR9	S_Shore
	cg20728490	0.032	0.37	0.049	0.026	DNTT	OpenSea
	cg12053348	0.036	0.61	0.056	0.026	NA	OpenSea
	cg03380960	0.019	0.48	0.045	0.034	FAM53B	OpenSea
	cg26274304	0.018	0.36	0.027	0.037	NCAPG2	N_Shore
	cg16730908	0.021	0.39	0.032	0.037	PSTPIP2	S_Shore
	cg13904562	0.041	0.53	0.056	0.037	NA	OpenSea
	cg07508217	0.026	0.69	0.042	0.037	NA	OpenSea
	cg03516318	0.028	0.21	0.039	0.037	MBIP	OpenSea
	cg10883621	0.02	0.35	0.032	0.037	C2orf39	Island
	cg22402007	0.022	0.16	0.031	0.041	NTRK2	N_Shore
	cg11380624	0.024	0.27	0.036	0.041	DNAJC14	N_Shore
	cg15882605	0.025	0.44	0.051	0.041	NA	OpenSea
	cg03818156	0.017	0.9	0.028	0.041	NA	OpenSea
	cg13288863	0.02	0.79	0.049	0.048	CDO1	N_Shore
	cg03743584	0.018	0.3	0.025	0.048	PRAP1	OpenSea
	cg10981514	0.023	0.42	0.042	0.048	TPCN1	OpenSea
	cg06600694	0.005	0.06	0.008	0.048	IRS1	Island
	cg14700085	0.016	0.71	0.024	0.050	CSF1R	OpenSea

A for father’s any preconception smoking, in the full cohort (N = 875), and B for father’s smoking starting before age 15 years, in the subpopulation (N = 304)
*Coefficient: Regression coefficient between father smoking/not smoking
**Average methylation across all samples
***adj. P. Val: FDR adjusted p value
****N (north) Shelf: up to 2 kb outward from flanking shores; OpenSea: > 4 kb from CpG islands; N (north) and S (south) Shores: up to 2 kb from flanking CpG islands

ISSN: 1868-7083