Abnormal DNA methylation within genes of the steroidogenesis pathway two years after paediatric critical illness and association with stunted growth in height further in time

Vanhorebeek, Ilse; Coppens, Grégoire; Güiza, Fabian; Derese, Inge; Wouters, Pieter J.; Joosten, Koen F.; Verbruggen, Sascha C.; Van den Berghe, Greet

doi:10.1186/s13148-023-01530-9

Table 2 Abnormal DNA methylation within the steroidogenesis pathway

From: Abnormal DNA methylation within genes of the steroidogenesis pathway two years after paediatric critical illness and association with stunted growth in height further in time

Gene	CpG site	Gene section^a,b	Methylation status	Former PICU patients vs healthy children					Former PICU patients: GC vs No GC treatment P-value^e
				Log fold change [confidence interval]^c	Absolute mean difference^d	P-value^e
				Log fold change [confidence interval]^c	Absolute mean difference^d	Patients vs healthy children ^f	Interaction with sex	Interaction with age
CYP11A1	cg23808031	Promoter/1stExon	Hypermethylated	0.195 [0.090 to 0.301]	0.041	0.016	0.51	0.82	0.0090
POR	cg10738873	5'UTR	Hypomethylated	−0.116 [−0.175 to −0.058]	0.011	0.0092	0.69	0.26	0.22
	cg17115737	5'UTR	Hypomethylated	−0.099 [−0.159 to −0.039]	0.026	0.036	0.49	0.84	0.84
	cg27372063	Body	Hypomethylated	−0.182 [−0.286 to −0.078]	0.026	0.026	0.29	0.24	0.068
CYB5A	cg18274065	Promoter	Hypomethylated	−0.133 [−0.196 to −0.071]	0.019	0.0067	0.32	0.79	0.18
HSD17B1	cg02363277	Body	Hypermethylated	0.039 [0.015 to 0.062]	0.002	0.044	0.80	0.61	0.34
HSD17B2	cg09894383	Promoter	Hypomethylated	−0.080 [−0.126 to −0.035]	0.023	0.026	0.30	0.012	0.98
	cg11515282	Promoter	Hypomethylated	−0.100 [−0.148 to −0.052]	0.023	0.0067	0.76	0.029	0.52
	cg20373326	Promoter	Hypomethylated	−0.135 [−0.190 to −0.080]	0.027	0.0009	0.84	0.36	0.34
	cg19807685	5'UTR/1stExon	Hypomethylated	−0.124 [−0.182 to −0.065]	0.011	0.0067	0.47	0.18	0.84
	cg26315602	1stExon	Hypomethylated	−0.073 [−0.113 to −0.034]	0.010	0.016	0.76	0.93	0.66
	cg00365986	Body	Hypomethylated	−0.134 [−0.212 to −0.057]	0.016	0.026	0.99	0.25	0.045
	cg05315365	Body	Hypomethylated	−0.170 [−0.259 to −0.082]	0.046	0.013	0.55	0.017	0.23
	cg13740036	Body	Hypomethylated	−0.192 [−0.287 to −0.098]	0.052	0.0073	0.46	0.045	0.68
HSD17B3	cg18014207	Body	Hypomethylated	−0.131 [−0.204 to −0.058]	0.031	0.025	0.89	0.13	0.056
HSD17B6	cg21922731	5'UTR/1stExon	Hypomethylated	−0.044 [−0.069 to −0.018]	0.010	0.026	0.44	0.87	0.68
HSD17B10	cg26323797	Promoter	Hypermethylated	0.146 [0.062 to 0.231]	0.024	0.026	0.47	0.32	0.18
HSD17B12	cg14262884	Body	Hypomethylated	−0.081 [−0.131 to −0.030]	0.010	0.049	0.38	0.048	0.66
	cg21077321	Body	Hypomethylated	−0.133 [−0.212 to −0.055]	0.025	0.031	0.86	0.081	0.68
CYP19A1	cg12009872	Body	Hypomethylated	−0.057 [−0.092 to −0.021]	0.015	0.046	0.93	0.012	0.67
	cg14424631	Body	Hypomethylated	−0.093 [−0.138 to −0.048]	0.020	0.0072	0.85	0.035	0.0027
CYP21A2	cg11675917	Promoter	Hypomethylated	−0.129 [−0.199 to −0.060]	0.019	0.016	0.043	0.24	0.44
CYP11B2	cg14389499	Promoter	Hypomethylated	−0.100 [−0.159 to −0.041]	0.031	0.031	0.88	0.41	0.14

GC glucocorticoid; PICU paediatric intensive care unit, UTR untranslated region
^aA CpG site can be located within multiple splice variants and thus can be situated within multiple gene sections
^bPromoter is defined as 0 to 1500 base pairs upstream of the transcription start site
^cLog fold change in M-values between former PICU patients and healthy controls adjusted for risk factors with 95% confidence interval
^dThe absolute mean difference is the (unadjusted) absolute difference between the mean beta-value of a given CpG within the Former PICU patients and the mean beta-value of a given CpG within the healthy control children
^eP-values from multivariable models, adjusted for baseline risk factors and technical variation. All P-values come from separate models. Numbers in bold indicate a significant P-value
^fAdjusted for multiple testing using a false discovery rate < 0.05

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