Fig. 7

Physical exercise restores paraspinal muscle asymmetry. A Concave muscle activation was stretched after Schroth exercises. B Representative immunohistochemical images of type I muscle fiber (green) in paraspinal muscle from AIS patients who performed Schroth exercises longer than 3 months. C Immunofluorescence showed that the percentage of type I muscle fiber was comparable between convex and concave sides. n = 5 in each case; data are represented as mean ± s.d. Scale bar, 100 μm. D Quantitative real-time PCR analysis showed that MYH7, MYHB, MYH1, and TNNI2, which had an asymmetric expression in untrained AIS, were restored. n = 5 in each case; data are represented as mean ± s.d. E Representative Western blot for FTO expression between convex and concave sides. F Western blot quantification showed no significant difference in FTO expression between convex and concave sides. n = 5 in each case; data are represented as mean ± s.d. G The m⁶A level was comparable between convex and concave paraspinal muscle. n = 5 in each case; data are represented as mean ± s.d. *P < 0.05