Fig. 6

Reduced FTO in the concave paraspinal muscle of congenital scoliosis. A Magnetic resonance imaging showed convex and concave curves of congenital scoliosis, and the yellow arrow showed hemivertebra deformity. B Representative immunohistochemical images of MHC1 (green) in paraspinal muscle. Scale bar, 100 μm. C Immunofluorescence showed that the percentage of type I muscle fiber was significantly reduced in concave paraspinal muscle. n = 5 in each case; data are represented as mean ± s.d. D Quantitative real-time PCR analysis showed that mRNA expression of MYH7 and MYH7B was decreased, but MYH1 and TNNC2 mRNA levels were increased in concave paraspinal muscle. n = 5 in each case; data are represented as mean ± s.d. E The m⁶A level was significantly increased in concave paraspinal muscle. n = 5 in each case; data are represented as mean ± s.d. F Representative Western blot of FTO in paraspinal muscle. G Western blot quantification showed that FTO was significantly decreased in concave paraspinal muscle. n = 5 in each case; data are represented as mean ± s.d. H Quantitative real-time PCR analysis showed that FTO mRNA expression was decreased in concave paraspinal muscle. n = 5 in each case; data are represented as mean ± s.d. *P < 0.05, **P < 0.01