Fig. 2
From: FTO-dependent m6A regulates muscle fiber remodeling in an NFATC1–YTHDF2 dependent manner
Fto maintains expression of murine slow-twitch fiber related genes in vitro. A During myogenic differentiation, the mRNA expression of Fto was gradually increased, and the m6A level gradually decreased. n = 5 in each case; data are represented as mean ± s.d. B Quantitative real-time PCR analysis showed that the expression of m6A-related genes was undisturbed after Fto knock-down. n = 5 in each case; data are represented as mean ± s.d. C Representative immunohistochemical images of MHC1 (red) during myogenic differentiation. Scale bar, 100 μm. D Immunofluorescence revealed that the MHC1 fusion index was significantly decreased in si-Fto treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. E Immunofluorescence revealed that myotube width was significantly decreased in si-Fto treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. F Representative immunohistochemical images of MyoG (green) during myogenic differentiation. Scale bar, 100 μm. G Immunofluorescence revealed no significant difference for the MyoG-positive nucleus index in si-Fto treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. H Quantitative real-time PCR analysis showed that the expression of myogenic regulatory factors was undisturbed after Fto knock-down. n = 5 in each case; data are represented as mean ± s.d. I Quantitative real-time PCR analysis revealed that si-Fto interference restrained the expression of slow-twitch fiber related genes, including Myh7 and Myh7b. n = 5 in each case; data are represented as mean ± s.d. J Myh7 mRNA stability showed no difference in si-Fto treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. K Myh7b mRNA stability showed no difference in si-Fto treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001,#P < 0.05, ##P < 0.01