Fig. 1
From: FTO-dependent m6A regulates muscle fiber remodeling in an NFATC1–YTHDF2 dependent manner
Fto regulates the proliferation and migration of murine myoblasts. A Representative Western blot analysis of si-Fto interference in C2C12 cells. B Western blot quantification verified the knock-down efficiency of si-Fto interference. n = 3 in each case; data are represented as mean ± s.d. C Quantitative real-time PCR analysis verified the knock-down efficiency of si-Fto interference. n = 5 in each case; data are represented as mean ± s.d. D The m6A level was significantly increased after si-Fto interference. n = 5 in each case; data are represented as mean ± s.d. E Immunofluorescence staining of Ki67 after si-Fto interference in myoblasts. Scale bar, 50 μm. F Interference with si-Fto decreased the percentage of Ki67-positive cells. n = 5 in each case; data are represented as mean ± s.d. G Cell count analysis showed consistent trends with immunofluorescence analysis of Ki67. n = 5 in each case; data are represented as mean ± s.d. H Representative images of the live cell tracking technique. Scale bar, 200 μm. I The accumulated distance was significantly decreased in si-Fto-treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. J Displacement per track was significantly decreased in si-Fto treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. K Average speed per track was significantly decreased in si-Fto treated myoblasts. n = 5 in each case; data are represented as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001