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Fig. 2 | Clinical Epigenetics

Fig. 2

From: CTLA4 DNA methylation is associated with CTLA-4 expression and predicts response to immunotherapy in head and neck squamous cell carcinoma

Fig. 2

Pharmacological demethylation decreases CTLA4 methylaton and induces CTLA-4 expression in head and neck squamous cell carcinoma (HNSCC) cells a CTLA4 mRNA expression (ΔCT levels) in 5‐aza‐dC treated (+ Aza) compared to untreated (− Aza) HNSCC cells and six individual tonsils. b CTLA-4 expression (mean fluorescence intensity [MFI]) of 5-aza-dC treated (+ Aza) compared to untreated (− Aza) HNSCC cell lines. c CTLA4 Methylation [QMS] of 5-aza-dC treated (+ Aza) compared to untreated (− Aza) HNSCC cell lines. d, e Normalized histograms (d) and IHC (e) illustrating induction of CTLA-4 protein expression in pharmacologically demethylated (+ Aza) compared to untreated (− Aza) HNSCC cell lines. Bars represent mean values. P-values refer to Kruskal–Wallis and t-tests, respectively

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Clinical Epigenetics

ISSN: 1868-7083

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