Fig. 2
From: Quantification of hematopoietic stem and progenitor cells by targeted DNA methylation analysis
DNA methylation in subsets of hematopoietic progenitor cells. A Candidate CpGs were selected for hematopoietic stem cells (HSCs), lymphoid-primed multipotent progenitor (LMPPs), and common myeloid progenitor cells (CMPs). The selection is based on (1) difference of mean β-value in DNAm profiles of these progenitor subsets (GSE63409) in comparison with all other subsets and leukocytes (GSE35069) and (2) variance of β-values within these groups. Three hypo- (blue) and three hypermethylated CpGs (red) were depicted for each subset. B Gene expression profiles were compared in HSPCs versus leukocytes (GSE24759), and 12 of the 18 selected CpGs were significantly differentially expressed (adjusted P value < 0.05). C Heatmap depicts DNAm (β-values) for 18 CpGs (GSE63409, GSE35069). D Estimation of HSPC subsets based on a non-negative least square model (NNLS model) for 6 CpGs in different types of cryopreserved blood samples. E Pearson correlation of DNAm levels in pyrosequencing with CD34 counts in mPB (HSPCs, flow cytometry, n = 9) and leukemic blasts (manual counts, n = 39)