Fig. 3
From: Epigenome-wide association study identifies novel genes associated with ischemic stroke
Validation of the CRISPR/dCas9-Dnmt3a model construction in human umbilical vein endothelial cells (HUVEC). Pyrosequencing was used to measure DNA methylation levels in the engineered and control cells. Successful model construction was considered when DNA methylation level of the engineered cells was higher than that of the negative control (i.e., TRIM6, FLRT2, and TLN2 genes). No significant differences in DNA methylation levels were observed for SOX1, FAM84A, SOX17, and AGBL4 genes