Fig. 2
From: Buffy coat signatures of breast cancer risk in a prospective cohort study
Differentially methylated sites detected in prospectively collected blood samples from individuals who develop cancer within the study timeframe when compared with age- and lifestyle-matched control individuals. a Volcano plot illustrating results of differential methylation analyses comparing regions annotated to promoters between case and control buffy coat samples. b Manhattan plot illustrating the chromosomal locations of the top differentially methylated sites between cases and controls. Scatterplots demonstrating a positive correlation between DNA methylation levels at c SURF6 and d REXO1/CTB31O20.3 and length of time to diagnosis suggest that progressive demethylation at these sites could be indicative of early carcinogenesis. CpG sites that were found to be driving this relationship are highlighted in the regional heat map on the right