Fig. 2
Identification of biomarkers for the HCC diagnostic model development by qMSP. A Methylation levels of 10 candidate methylated CpG sites as quantified by qMSP in 60 pairs of HCC and normal tissues. The y-axis represents methylation levels (ΔCt = Ctreference = Cttarget), in which a lower value represents a relatively higher methylation level. ****p < 0.0001. B Area under the ROC curve (AUC) of eight biomarkers in diagnosing HCC from normal plasma samples. Markers that have AUCs of > 0.8 and < 0.8 are indicated in red and blue, respectively. C ROC curves for 4 different biomarker panels in the plasma pilot study. The 4 panels were as follows: 3 methylated CpG sites (cg14263942, cg12701184, and cg14570307), 3 methylated CpG sites with AFP and DCP, 6 methylated CpG sites (cg14263942, cg12701184, cg14570307, cg15457058, cg07689503, and cg20172627), and 6 methylated CpG sites with AFP and DCP. The AUC values for each curve are included in parentheses