Fig. 2

Manhattan plot of the epigenetic loci in granulosa cells that are differentially methylated with high versus low supplemental folic acid intake. The x-axis shows the genomic location of the individual CpG sites. The y-axis shows the − log₁₀(raw p-values) from models relating high supplemental folic acid intake to CpG methylation, adjusting for age and three surrogate variables. The horizontal line depicts the Bonferroni adjusted p-value threshold. Blue dots indicate a positive association between supplemental folic acid intake and DNA methylation and red dots indicate a negative association between supplemental folic acid intake and DNA methylation. Significant findings after adjustment for multiple comparison were annotated with CpG ID (closest gene)