Fig. 5
From: CRISPR-Cas9 knockout screen identifies novel treatment targets in childhood high-grade glioma
Knockout induces alterations in stem cell and differentiation markers. A Knockout of the candidate genes leads to decreased proliferation (EdU) and expression of stem cell marker SOX2, and increase in the differentiation marker GFAP. An asterisk (*) under a bar denotes significant difference of the marker compared to the negative control for the cell line (Welch one-sided t test, p value < 0.05). For example, knockout of EED in GU-pBT-7 leads to significantly decreased proliferation rate (EdU) and significantly increased expression of GFAP compared to the negative control of GU-pBT-7, while the decrease in SOX2 is not statistically significant (p value = 0.065). Error bars represent the standard error mean (n = 2 technical replicates per knockout condition and staining. EdU ctrl n = 12 technical replicates, GFAP and SOX2 ctrl n = 4 technical replicates). B A large increase in GFAP was in particular seen for CHD4 and UBE2N knockout, respectively. Scale bar = 100 µm applies to all images