Fig. 1
From: CRISPR-Cas9 knockout screen identifies novel treatment targets in childhood high-grade glioma
Screen library and hit calling. A Epifactor classification of the genes in the screen. B Average rank (of technical duplicates) for GU-pBT-7 vs GU-pBT-19 in the knockout screen. Lower rank indicates more essential/depleted after knockout. We set a threshold at ranking ≤ 250 for hit calling, with at least 3 (out of 4) concordant gRNA and replicate overlap. C These criteria resulted in 154 hits, where 44 were shared between the cell lines. D The significantly enriched GO terms for the hits (compared to the screen background) are presented