Fig. 8

Dynamic sites in the genome are susceptible to ART/hypofertility-induced methylation defects. A Proportion of environmentally sensitive dynamic sites among DMCs between ART and control groups when all, only male and only female samples were used for comparison. Chi-square with Yate’s correction was used to compare the proportion of dynamic sites among DMCs relative to all captured sites genome-wide; ***p < 0.001. B Venn diagram exclusively showing dynamic DMCs commonly affected by ART using all, only male and only female samples. C Significant biological processes from Gene Ontology analysis on all dynamic DMCs annotated to genic regions for the ART versus Control analysis in males and females. The selected biological processes shown were also significantly enriched when all DMCs (dynamic and non-dynamic) were included in the analysis. The number of observed genes associated with each term is indicated within bars. Enrichment is defined as the proportion of observed genes compared to the number annotated within the whole dataset and is indicated by bar colour. The dotted line represents the significance threshold: weighted Fisher’s p value < 0.01