Table 3 Antibody dilutions for western blot and immunofluorescent staining of human pancreatic ductal epithelial cells
Antibody | Dilution |
|---|---|
Rabbit H3K27me3 | 1:2500 |
Mouse total H3 | 1:1000 |
Rabbit H3K27ac | 1:1000 |
Mouse β-actin | 1: 10,000 |
Rabbit EZH2 | 1:1000 |
IRDye® 680CW goat anti-rabbit | 1:10,000 |
IRDye® 800CW goat anti-mouse | 1:10,000 |
Rabbit anti-CK19 | 1:200 |
Guinea pig anti-insulin | 1:250 |
Alexa fluor 488 donkey anti-rabbit | 1:1000 |
IRDye® 680CW donkey anti-guinea pig | 1:1000 |